Searchable abstracts of presentations at key conferences in endocrinology

ea0070aep1029 | Hot topics (including COVID-19) | ECE2020

EndogenousInterleukin production by the skin cells (keratinocytes and fibroblasts) cocultured with mononuclears of patients with type 2 diabetes mellitusand type 2 diabetes with chronic foot ulcers in a 3D culture model

Mashkova Maryia , Mokhort Tatsiana , Goranov Vitaly

Our research focused on the role of immune mechanisms in diabetic foot ulcers development and impaired healing. The AIM of this study was to evaluate endogenous interleukin (IL1,4,6,8) production to the culture medium by the skin cells (keratinocytes and fibroblasts) cocultured with mononuclears of patients with type 2 diabetes mellitus (T2DM) and T2DM with chronic foot ulcers (DFU) in a 3D culture model.Materials and methods: A multilayer 3D immunocompe...

ea0063gp33 | Diabetes and Cardiovascular Disease | ECE2019

Application of 3D skin culture model as a tool to study the role of immune mechanisms in chronic diabetic foot ulcers pathogenesis

Mashkova Maryia , Goranov Vitaly , Mokhort Tatiana , Shyshko Alena , Mantachik Marina

The aim: To test cytotoxic effects of immune factors of patients with chronic diabetic foot ulcers on keratinocytes and fibroblasts in a 3D skin culture system.Materials and methods: In this study a multilayer 3D human skin model comprising of keratinocytes, fibroblasts and dendritic cells in an agarose-fibronectin gel was used. 20% serum of 13 patients with chronic noninfected diabetic foot ulcers, 13 diabetic type 2 patients and 13 healthy people and l...