ea0063gp33 | Diabetes and Cardiovascular Disease | ECE2019
Mashkova Maryia
, Goranov Vitaly
, Mokhort Tatiana
, Shyshko Alena
, Mantachik Marina
The aim: To test cytotoxic effects of immune factors of patients with chronic diabetic foot ulcers on keratinocytes and fibroblasts in a 3D skin culture system.Materials and methods: In this study a multilayer 3D human skin model comprising of keratinocytes, fibroblasts and dendritic cells in an agarose-fibronectin gel was used. 20% serum of 13 patients with chronic noninfected diabetic foot ulcers, 13 diabetic type 2 patients and 13 healthy people and l...